Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Polarization Filters, 535 nm, Polarized
Molecular Devices fluorescence polarization filter set designed for fluorescence polarization (FP) assays on compatible SpectraMax and FlexStation multi-mode microplate readers. The set contains polarized filters in vertical and horizontal orientations centered at 535 nm with a 25 nm bandwidth to support accurate FP measurements.
- Enables fluorescence polarization measurements.
- Includes vertical and horizontal polarized filters.
- Center wavelength 535 nm, bandwidth 25 nm.
- Includes two filters for polarization detection.
- Designed for compatible multi-mode microplate readers.
Molecular Devices Absorbance Filter, 280 nm, 5.5 nm Bandwidth
Molecular Devices 280 nm absorbance filter designed as a replacement slide-style optical filter for select multimode microplate readers. The narrow 5.5 nm bandwidth enables precise absorbance measurements at 280 nm for protein quantitation and other absorbance-based assays.
- Provides narrowband absorbance detection at 280 nm with a 5.5 nm bandwidth.
- Optimized for protein quantitation and small-volume absorbance assays.
- Slide-style form factor suitable for insertion into accessory filter slots.
- Low stray light and high transmission within the passband.
- Intended as a replacement optical filter to restore instrument performance.
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Vertical
Fluorescence polarization filter element with a 625 nm center wavelength and 35 nm bandwidth, polarized vertical, designed as an accessory for compatible microplate readers to enable fluorescence polarization assays.
- 625 nm center wavelength.
- 35 nm bandwidth.
- Vertical polarization orientation.
- Designed for fluorescence polarization assays.
- Compatible with microplate reader accessory filter holders.
- Easy installation into the reader filter slot.
Molecular Devices Absorbance Filter, 480 nm, 8 nm, FilterMax
This replacement absorbance photometric filter is designed for use in Molecular Devices microplate readers and provides narrowband absorbance detection centered at 480 nm for photometric assays that require precise wavelength selection.
- Provides narrowband absorbance detection centered at 480 nm.
- 8 nm full width at half maximum bandwidth for selective measurements.
- Intended for absorbance and photometric microplate assays.
- Compatible with microplate reader filter holders used in multi-mode readers.
- Useful for assays requiring blue-green wavelength detection.
Molecular Devices Absorbance Filter, 515 nm, 8 nm
Absorbance bandpass filter suitable for microplate absorbance assays. Centered at 515 nm with an 8 nm bandwidth, this optical filter installs in filter slide slots and provides narrowband absorbance detection for plate-based assays. Compatible with FilterMax F3 and F5 multimode microplate readers.
- Center wavelength 515 nm.
- Bandwidth 8 nm.
- Filter type Absorbance bandpass.
- Application Microplate absorbance measurements.
- Form factor Slide-mounted filter for filter-based readers.
Ace Glass, Inc. 100mL vessel with sloped shoulders and a #25 Ace-Thred neck
100mL vessel with sloped shoulders and a #25 Ace-Thred neck; includes PTFE lined cap. For use in freeze drying and storage. After drying material, vessel can be capped and stored. Working capacity of vessel is about 1/2 of volume.
Molecular Devices Photometric Filter, Multimode, Compatible with FilterMax F3, F5
AD 340 photometric filter from Molecular Devices is an accessory designed for multimode microplate readers; it provides wavelength selection for photometric assays and is intended for microplate and ELISA applications.
- Compatible with FilterMax F3 and FilterMax F5 multimode microplate readers.
- For photometric measurements on microplates and ELISA assays.
- Sold individually, quantity per sell 1.
- Country of origin Austria.
Molecular Devices Fluorescence Filter, Holder, 360 nm, BW 35 nm
Fluorescence filter with holder for installation in compatible microplate readers. Provides excitation in the near-UV at a 360 nm center wavelength with a 35 nm bandwidth; supplied with a holder for secure mounting and intended for fluorescence-based microplate assays.
- Provides 360 nm center wavelength excitation.
- 35 nm bandwidth for selective spectral isolation.
- Supplied with holder for microplate reader installation.
- Designed for fluorescence detection in microplate assays.
- Suitable for assays requiring near-UV excitation and narrow-band filtering.
Molecular Devices Microplate Reader, Multi-Mode, Tunable Monochromator, 6-384 Well
The SpectraMax iD3 multi-mode microplate reader from Molecular Devices provides absorbance, fluorescence (top and bottom), and luminescence detection for 6-384 well microplates. It features a tunable monochromator with 1-nm increments, intuitive touchscreen control, and software tools for spectral scanning and assay optimization, enabling rapid assay development and reliable quantitative measurements.
- Multi-mode detection for absorbance, fluorescence, and luminescence assays.
- Tunable monochromator with 1-nm wavelength increments for spectral flexibility.
- Broad wavelength coverage for diverse assay types, including UV and visible ranges.
- Supports 6 to 384 well plate formats for assay throughput flexibility.
- Intuitive touchscreen and software tools for quick setup and assay optimization.
- High photometric precision and repeatability for reliable quantitative results.
Molecular Devices Enhanced TRF Module, Time-Resolved Fluorescence, SpectraMax iD5 Compatible
The Enhanced TRF Module expands time-resolved fluorescence performance of SpectraMax iD5 and iD5e microplate readers, enabling TRF, TR-FRET, HTRF, IMAP, and ScanLater western blot reads with improved sensitivity for lanthanide assays.
- Improved sensitivity for Europium detection down to 10 fM.
- Enables TRF, TR-FRET, and HTRF assay read modes.
- Supports ScanLater western blot TRF reads.
- Operates with configurable flash intervals for time-resolved reads.
- Compatible with exchangeable excitation and emission filter slides sold separately.
- Requires compatible data acquisition software for some TR-FRET read modes.
Molecular Devices Multi-Mode Microplate Reader, Dual Monochromator, 6-384-Well
The SpectraMax M3 multi-mode microplate reader is a configurable, high-performance instrument for absorbance, fluorescence, and luminescence assays, designed for both research and regulated laboratory environments. It combines tunable optics with flexible plate handling and compliance-capable analysis software.
- Dual monochromator optics, tunable in 1-nm increments
- Detection modes include absorbance, fluorescence, luminescence, fluorescence polarization, and time-resolved assays
- Supports 6- to 384-well microplates, with top and bottom reading options
- High-sensitivity detectors with xenon flash lamp illumination
- Software features secure data acquisition and 21 CFR Part 11-compatible controls
Molecular Devices Microplate Reader, 6-1536-Well, Dual PMT, User-Upgradeable
The SpectraMax Paradigm multi-mode microplate reader from Molecular Devices is a user-upgradeable detection platform for absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization, and luminescence assays. It supports 6- to 1536-well plates and modular detection cartridges for configurable optics.
- User-upgradeable detection cartridges for configurable optics and modes.
- Supports 6- to 1536-well plate formats.
- Dual photomultiplier tubes for enhanced sensitivity and throughput.
- Wide absorbance range 230-1000 nm and fluorescence excitation/emission coverage.
- Integrated temperature control, plate shaking, and robotic compatibility.
- High dynamic range greater than six decades for quantitative assays.
- Compact footprint: 39 cm W x 45 cm H x 63 cm D.